Identification of Auto-antibody Producing Cells by Observing Antigen-antibody Reaction on the Cell Surface

Studies on Antibody-producing Cells

A study of the kinetics of antibody-producing cells has been carried out by the use of rosette formation for detection of individual antibody-producing cells, and labeling with tritiated thymidine, in cells obtained from mouse spleens at intervals after injection of SRBC. Following a primary injection of the antigen, the number of RFC per million cells was found to increase to a peak at 5 days,...

Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung

Background: Lung carcinoma is a multiple type cancer comprising of small cell and non-small cell carcinomas (NSCLC). For therapeutic and diagnostic purposes, serum monoclonal antibodies have been produced against lung cancer. Objective: To charac-terize a murine monoclonal antibody (ME3D11) reactive with human NSCLC. Methods: A murine monoclonal antibody (ME3D11) reactive with human NSCLC was s...

Factors affecting the antigen-antibody reaction.

Types of weak interactions According to quantum mechanics, all chemical bonds are based on electrostatic forces. A list of types of weak bonds is shown in table I, together with their strength (energy). Van der Waals forces are the weakest, but they are able to attract all kinds of molecules. Hydrogen or iondipole bonds require oppositely charged atoms. Hydrogen bonds are very important in aque...

The antibody-antigen reaction: a visual observation.

A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6.

In an attempt to identify B cell specific antigens, we have generated a mouse monoclonal antibody, R2D6, which is directed against plasma membranes of rat pancreatic B cells but against no other pancreatic cells. R2D6 crossreacted with mouse and guinea pig B cells, but not with human or dog. The B cell specificity of R2D6 was utilized in fluorescence-activated cell sorting to prepare highly enr...